The online measurement of dissolved oxygen concentration and pH in shaken bioreactors paves the way for proper scale up or scale down activities - from bench-top stirred bioreactors to larger or smaller scales. So far, appropriate measurement methods for culture monitoring in small scale cultivation vessels were missing. With the application of single-use optical sensors culture conditions can be monitored online without sampling. They enable highly parallelized measurements in multiwell plates, cell culture tubes, and differently designed flasks.
The non-invasive optical oxygen sensors measure the partial pressure of both dissolved and gaseous oxygen. They are fixed on the inner surface of the transparent glass or plastic material. The oxygen concentration can then be measured in a contactless and non-destructive manner from outside, through the wall of the vessel. Different coatings for different concentration ranges are available. Oxygen probes measure the partial pressure of both dissolved oxygen and gaseous oxygen. The oxygen sensor coating is integrated into high grade stainless steel fittings. The optical oxygen probes show an excellent long-term stability and stand rough process conditions.
Our systems combine oxygen and pH sensor probes and appropriate meters, and fit in nearly all standard shakers or incubators. Additionally, the SFR vario can also measure biomass and optical density. All our systems are mainly developed for industrial and routine research applications as well as parallel online monitoring and are, therefore, ideally suited for biotech & pharma applications.
Oxygen microsensors are miniaturized chemical optical oxygen sensors designed for all research and packaging applications where a small tip size (< 50 µm) and fast response time (t90 < 3 sec.) are necessary. The optical oxygen microsensors are based on 230 µm silica fiber and are available with sensor tip diameters from < 50 µm to 230 µm. The oxygen microsensors are mounted in different housings (needle-type housing, implantable, micro-profiling) and offer a unique research tool for investigating systems where micro-invasive and small sensors are needed.
Unwanted oxygen taken up during production or the filling process can shorten the shelf life of certain products. PreSens offers several sensor solutions for measurements in the final product under real conditions. Systems for measurements in the headspace and final product are offered.
Bags and single-use bioreactors are in the process of revolutionizing the way biopharmaceuticals are manufactured. Our non-invasive oxygen and pH sensors - integrated in the culture vessels - are the tools to turn simple disposables into full bioreactors. The ports are made of polyethylene which allows easy welding with cultivation bags.
The non-invasive optical sensors measure either the partial pressure of dissolved and gaseous oxygen, pH without the need for at-line sampling, or the CO2 concentration. They are fixed on the inner surface of the transparent glass or plastic material. The concentration can then be measured in a contactless and non-destructive manner from outside, through the wall of the vessel. For oxygen, different coatings for different concentration ranges are available.
Oxygen probes measure the partial pressure of both dissolved and gaseous oxygen. The oxygen sensor coating is integrated into high grade stainless steel fittings. The optical oxygen probes show an excellent long-term stability and stand rough process conditions. PreSens oxygen probes operate in industrial process control and various research applications. Certain probe models - equipped with standardized threads - are compatible with most bioreactors and port adapters and can be applied for dissolved oxygen measurements in large-scale cell or microbial cultures.